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TRP channels [electronic resource] / edited by Michael X. Zhu.

Contributor(s): Material type: TextTextSeries: Methods in signal transductionPublication details: Boca Raton : CRC Press, 2011.Description: xxii, 473 p., [4] p. of plates : ill. (some col.)ISBN:
  • 9781439818619 (ebook : PDF)
Subject(s): Genre/Form: Additional physical formats: No titleOnline resources: Available additional physical forms:
  • Also available in print edition.
Contents:
ch. 1. High-throughput screening of TRPC channel ligands using cell-based assays / Melissa Miller ... [et al.] -- ch. 2. Methods to study TRPC channel regulation by interacting proteins / Kirill Kiselyov ... [et al.] -- ch. 3. Activation of TRP channels in mammalian systems / Tomohiro Numata ... [et al.] -- ch. 4. Studying subunit interaction and complex assembly of TRP channels / Michael Poteser and Klaus Groschner -- ch. 5. Proteomic analysis of TRPC channels / Timothy Lockwich ... [et al.] -- ch. 6. Lessons of studying TRP channels with antibodies / Marcel Meissner ... [et al.] -- ch. 7. Assessing TRPC channel function using pore-blocking antibodies / Shang-Zhong Xu -- ch. 8. Strategies and protocols to generate mouse models with targeted mutations to analyze TRP channel functions / Marc Freichel ... [et al.] -- ch. 9. Studying endogenous TRP channels in visceral and vascular smooth muscles / Alexander V. Zholos --
ch. 10. TRPC channels in neuronal survival / Junbo Huang ... [et al.] -- ch. 11. Electrophysiological methods for the study of TRP channels / Karel Talavera and Bernd Nilius -- ch. 12. High-throughput approaches to studying mechanisms of TRP channel activation / Hongzhen Hu ... [et al.] -- ch. 13. Methods used for studying TRP channel functions in sensory neurons / Louis S. Premkumar -- ch. 14. Time-resolved activation of thermal TRP channels by fast temperature jumps / Feng Qin -- ch. 15. Fluorescence Microscopy in thermoTRP channel research / Fan Yang and Jie Zheng -- ch. 16. Regulation of TRP channels by osmomechanical stress / Min Jin ... [et al.] -- ch. 17. Study of TRP channels in cancer cells / V'yacheslav Lehen'kyi and Natalia Prevarskaya -- ch. 18. In vitro and in vivo assays for the discovery of analgesic drugs targeting TRP channels / Jun Chen ... [et al.] -- ch. 19. Studying TRP channels in intracellular membranes / Mohammad Samie and Haoxing Xu -- ch. 20. Methods for studying Drosophila TRP channels / Elkana Kohn and Baruch Minke -- ch. 21. Studying TRP channels in Caenorhabditis elegans / Rui Xiao and X.Z. Shawn Zu.
Summary: "Transient Receptor Potential (TRP) channels have gained a lot of popularity in recent years due to their involvement in a wide variety of cellular functions in many different systems. The purpose of this book is to provide detailed methods for the study of TRP channels. It includes expression and functional studies of TRP channels in heterologous systems as well as structural and functional studies of TRP channels. The author also explores various methods for detailed analysis of biophysical properties of TRP channels. In addition, the text covers high-throughput screening assays for TRP channels and offers novel and effective approaches to study endogenous TRP channel function in native systems"--Provided by publisher.
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"A CRC title."

Includes bibliographical references and index.

ch. 1. High-throughput screening of TRPC channel ligands using cell-based assays / Melissa Miller ... [et al.] -- ch. 2. Methods to study TRPC channel regulation by interacting proteins / Kirill Kiselyov ... [et al.] -- ch. 3. Activation of TRP channels in mammalian systems / Tomohiro Numata ... [et al.] -- ch. 4. Studying subunit interaction and complex assembly of TRP channels / Michael Poteser and Klaus Groschner -- ch. 5. Proteomic analysis of TRPC channels / Timothy Lockwich ... [et al.] -- ch. 6. Lessons of studying TRP channels with antibodies / Marcel Meissner ... [et al.] -- ch. 7. Assessing TRPC channel function using pore-blocking antibodies / Shang-Zhong Xu -- ch. 8. Strategies and protocols to generate mouse models with targeted mutations to analyze TRP channel functions / Marc Freichel ... [et al.] -- ch. 9. Studying endogenous TRP channels in visceral and vascular smooth muscles / Alexander V. Zholos --

ch. 10. TRPC channels in neuronal survival / Junbo Huang ... [et al.] -- ch. 11. Electrophysiological methods for the study of TRP channels / Karel Talavera and Bernd Nilius -- ch. 12. High-throughput approaches to studying mechanisms of TRP channel activation / Hongzhen Hu ... [et al.] -- ch. 13. Methods used for studying TRP channel functions in sensory neurons / Louis S. Premkumar -- ch. 14. Time-resolved activation of thermal TRP channels by fast temperature jumps / Feng Qin -- ch. 15. Fluorescence Microscopy in thermoTRP channel research / Fan Yang and Jie Zheng -- ch. 16. Regulation of TRP channels by osmomechanical stress / Min Jin ... [et al.] -- ch. 17. Study of TRP channels in cancer cells / V'yacheslav Lehen'kyi and Natalia Prevarskaya -- ch. 18. In vitro and in vivo assays for the discovery of analgesic drugs targeting TRP channels / Jun Chen ... [et al.] -- ch. 19. Studying TRP channels in intracellular membranes / Mohammad Samie and Haoxing Xu -- ch. 20. Methods for studying Drosophila TRP channels / Elkana Kohn and Baruch Minke -- ch. 21. Studying TRP channels in Caenorhabditis elegans / Rui Xiao and X.Z. Shawn Zu.

"Transient Receptor Potential (TRP) channels have gained a lot of popularity in recent years due to their involvement in a wide variety of cellular functions in many different systems. The purpose of this book is to provide detailed methods for the study of TRP channels. It includes expression and functional studies of TRP channels in heterologous systems as well as structural and functional studies of TRP channels. The author also explores various methods for detailed analysis of biophysical properties of TRP channels. In addition, the text covers high-throughput screening assays for TRP channels and offers novel and effective approaches to study endogenous TRP channel function in native systems"--Provided by publisher.

Also available in print edition.

Mode of access: World Wide Web.

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